Figure 4

The effect of TRAIL expression in JB6-pcDNA5/FRT TRAIL cells on co-cultured Jurkat cells. A Phase-contrast-imaging of apoptotic Jurkat cell after 48 hours of co-culture with JB6-pcDNA5/FRT TRAIL. b Annexin V/Propidiumiodide dyed Jurkat cells after 48 hours of co-culture with JB6-pcDNA5/FRT TRAIL. Membrane fluorescence is characteristic for apoptosis. c Phase-contrast-imaging of a Jurkat cell after 48 hours of co-culture with JB6-pcDNA5/FRT CAT. d Annexin V/Propidiumiodide dyed Jurkat cell after 48 hours of co-culture with JB6-pcDNA5/FRT CAT. No membrane fluorescence could be detected. e Western blot stained with anti-Caspase 8 followed by alkaline phosphatase-conjugated secondary antibody and NBT/BCIP colour development on the blot. Lane 1: untreated Jurkat cells, Lane 2: Jurkat cells kept in co-culture with JB6pcDNA5/FRT CAT, Lane 3: Jurkat cells kept in co-culture with JB6pcDNA5/FRT CAT prestimulated with concanavaline A, Lane 4: Jurkat cells kept in co-culture with JB6pcDNA5/FRT TRAIL, Lane 5: Jurkat cells kept in co-culture with JB6pcDNA5/FRT TRAIL prestimulated with concanavaline A.