TRAIL expression of cell line JB6-pcDNA5/FRT TRAIL. The lacZeo expression cassette was exchanged for the full-length cDNA of TRAIL by FRT recombination. The resulting cell clone was examined for TRAIL expression. a Western Blot of the cellular lysates of JB6pcDNA5/FRT TRAIL and JB6pcDNA5/FRT CAT. Immunostaining was performed by treatment with anti-TRAIL followed by incubation with alkaline phosphatase conjugated secondary antibody and colour development of NBT/BCIP. b Indirect immunofluorescence of JB6pcDNA5/FRT TRAIL. Cells were fixated and incubated with anti-TRAIL either directed against the C terminus or directed against the N terminus. The intracellular domain is detected only if the cells are permeable.