Figure 3

Percentage of dead Jurkat cells after 48 hrs in co-culture with JB6pcDNA5/FRT TRAIL and CAT, respectively. Cells were either prestimulated with concanavaline A or left untreated. Cell viabiliy was measured by MTT assay while apoptosis was determined by the caspase substrat reaction Apo-One and the percentage of dead Jurkat cells was calculated. All experiments were carried out in triplicates at least three independent times and presented as means with standard deviation. (p < 0,05 for the difference between cells expressing CAT and TRAIL, respectively.)